Hormonal Regulation of Epithelial Sodium Channel Genes in the Caco-2 Cell Line ♦ 317
نویسندگان
چکیده
منابع مشابه
Hormonal Regulation of the Epithelial Sodium Channel ENaC
Despite large changes in water and salt intake, the kidney is able to maintain the extracellular osmolarity and volume within narrow margins (Verrey et al., 2000). Such fine control requires specific factors or hormones ; among them, aldosterone and antidiuretic hormone (ADH) (vasopressin in mammals) play the key role. The main effector for the sodium balance is the epithelial sodium channel ex...
متن کاملRole of SGK in hormonal regulation of epithelial sodium channel in A6 cells.
The purpose of this study was to examine the role of the serum- and glucocorticoid-induced kinase (SGK) in the activation of the epithelial sodium channel (ENaC) by aldosterone, arginine vasopressin (AVP), and insulin. We used a tetracycline-inducible system to control the expression of wild-type (SGK(wt)(T)), constitutively active (S425D mutation; SGK(S425D)(T)), or inactive (K130M mutation; S...
متن کاملInduction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line.
Complete disruption of the microtubular network by colchicine or nocodazole in Caco-2 intestinal epithelial cells results in the appearance of basolateral microvilli and brush border-containing intracellular vacuoles (vacuolar apical compartment: VAC). These vacuoles are surrounded by a terminal web, express apical markers and exclude basolateral markers. The vacuoles do not originate from inte...
متن کاملBasolateral secretion of kappa light chain in the polarised epithelial cell line, Caco-2.
The immunoglobulin kappa light chain is constitutively secreted in non-polarised cells. It is therefore unlikely to display any of the signals thought to be required for the selective delivery of proteins to the apical or basolateral borders of polarised epithelial cells. We have transfected the gene for the kappa light chain into a polarised epithelial cell line (Caco-2) and shown that it is s...
متن کاملRegulation of Epithelial Sodium Transport via Epithelial Na+ Channel
Renal epithelial Na+ transport plays an important role in homeostasis of our body fluid content and blood pressure. Further, the Na+ transport in alveolar epithelial cells essentially controls the amount of alveolar fluid that should be kept at an appropriate level for normal gas exchange. The epithelial Na+ transport is generally mediated through two steps: (1) the entry step of Na+ via epithe...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1998
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-199804001-00338